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What exactly is magnification? A basic definition of magnification is the ratio of the size of a specific feature of

an object or sample as seen in an image produced by an optical system to the actual size of the feature on

the object itself.

In digital imaging this can be defined as the ratio between the size of the object as shown on the monitor and the actual physical size of the object.

 

So in order to display the correct magnification, the size of the monitor also must be known.